In this test,water and ethanol extraction were detected by electrospray ionization mass spectrometry,and the fragment mechanism of compounds in extractions.The results showed that the extractions were suit to be detected by ESI in positive and negative ion modes,which indicated component of beet was very complex.In tandem mass spectra,the compounds in sugarbeet extractions easily lost small neutral molecules of H2O,CO2 and glucose,and those contained hydroxyl,carboxyl and glucose groups.The groups were important to explain anti-oxygen ability of the compounds,which was agreed with documents.It was reliable that oxidation resistant of sugarbeet is studied by using ESI-MSn.ESI-MS can be used to directly analyze sugarbeet extraction with high sensitivity and avoiding tedious and time-consuming chemical manipulations,so that it can be used to screen antioxidants in complicate system.