Construction and Toxicity Analysis of Recombinant Baculovirus AcMNPV-BmK IT-vcath

Recombinant baculovirus AcMNPV-BmK IT-vcath was constructed using Bac to Bac expression system.The insect neurotoxin gene BmK IT and baculovirus vcath gene were constructed into the genome of AcMNPV,under the control of strong promoter p10 and polh respectively.Toxicity show the median lethal concentration(LC50) of recombinant and wild viruses were 28 829.5(pfu/mL) and 33 114.5(pfu/mL) respectively.The LC50 value of recombinant virus was lower 13% than wild type virus.At the concentration of 2×106 pfu/mL,the median lethal time(LT50) of two viruses were for 3.5 d and 4.1 d respectively.The LT50 of recombinant virus had a decrease of 14.6% compared with wild type one.The results showed recombinant virus improved insecticidal potency and killing rate.