Anti-Proliferation Effect of Dicentrine on Human Lung Cancer Cell Line A549 and Its Mechanism

OBJECTIVE To investigate the inhibitory effect of dicentrine on human lung cancer cell line A549.METHODS MTT colormetric assay was used to examine the growth inhibition of dicentrine of 25,50,100,and 200 μmol·L-1on human lung cancer cell line A549.Moreover,TUNEL test was performed to observe the apoptosis induced by the dicentrine.The cell cycle of A549 cells treated with dicentrine was detected by flow cytometry.The activity of caspase-3 was determined by colorimetric assay.RESULTS The inhibitory effect of the dicentrine on A549 cells increased in a dose-and time-dependent manner.Dicentrine could significantly induce apoptosis of A549 cells.Compared with the control group,the number of A549 cells in S phase significantly increased in dicentrine group(P0.05).Caspase-3 was activated.CONCLUSION Dicentrine can significantly inhibit the proliferation of A549 cells and block the cell cycle at S phase,which induces the apoptosis of A549 cells.Caspase-3 is responsible for the apoptosis of A549 cell induced by dicentrine.