Simultaneous quantitative determination of four active components in Salvia miltiorrhiza tablets by HPLC

Aim:To develop a method for rapid and sensitive simultaneous determination of the hydrophilic and lipophilic active components in Salvia miltiorrhiza tablets.Methods:RP-HPLC condition was established to separate danshensu,salvianolic acid B,cryptotanshinone and tanshinone Ⅱ_A from each other on the Diamonsil C_18(250 mm×4.6 mm ID,5 μm)column with the mobile phase consisted of methanol and 0.5% formic acid in gradient elution.Results:The four active components had good linearity.The average recoveries ranged between 96.63% to 100.3% with RSDs of less than 2%.Conclusion:The method appeared to be precise and accurate for the simultaneous determination of the four active components in Salvia miltiorrhiza Bunge tablets within 35 min.