Primers Screening and Identification of Three Samples in Helianthus tuberosus L.by ISSR Molecular Marker

Genetic differences and kindredship among 3 samples in Helianthus tuberosus L.were studied by adopting ISSR markers.16 out of 100 ISSR primers were screened out and 160 ISSR markers were amplified from these clones with 132 polymorphic(percentage of polymorphic bands was 82.5%).The value of Neips genetic similarity(GS) indexes of 3 cultivars based on the ISSR data varied from 0.44 to 0.86,with an average of 0.3,suggesting that the phylogenetic relationships of three Helianthus tuberosus L.were different.Molecular identification card was established to distinguish the three Helianthus tuberosus L.This study would provided information for cultivar identification of Helianthus tuberosus L.