Relationship between the drug-resistance phenotype and its genotype of the extended spectrumβ-Lactamase Klebsiella Pneumoniae

【Objective】To analyze and study the drug resistance phenotype of Klebsiella Pneumoniae and its relationship with genotypes.To detect the epidemic situation of different ESBL genotype.【Method】Detected the drug resistance of Klebsiella Pneumoniae by K-B method.Studied the relationship between its drug-resistance phenotype and its genotype by PFGE method.Analyzed the epidemic situation of ESBL genotype by PCR amplifying TEM,SHV and CTX-M gene fragments.【Results】It had a much severer drug-resistance nowadays for Klebsiella Pneumoniae and the result of ESBL-producing rate was 43.59%.117 clinical strains were dispatched into three groups by PFGE and its similar value resulted in 37.30%.No special ESBL DNA band was found.SHV detection rate resulted in 29.4%(15/51) by PCR,TEM 76.5%(39/51),CTX-M17.6%(9/51).【Conclusion】Much severer drug resistance of Klebsiella Pneumoniae was identified and most of it appeared multi-drug resistance.ESBL detecting rate was rather high.No nosocomoal infection resulted by a strain was detected through the study.Some drug resistance phenotype of Klebsiella Pneumoniae can be in accord with its genotype,while some not.The major genotype of ESBL producing Klebsiella Pneumoniae were tested to be TEM and SHV and it was very common strain carried multi-drug resistance genes.