In view of these problems of the fine disseminated grain size of fluorite and quartz in fluorite ore in a certain place,the low recovery of fluorite and the poor quality of concentrate under the low temperature(15℃), the experiment adopted the process of one roughing one scavenging seven cleanings and concentrate of the first cleaning regrinding and re-flotation,and used self-developed ZYM as the collector of fluorite low temperature flotation. Under the pulp temperature of 5 ~10℃,the beneficiation indicators in the laboratory were as follows:fluorite ore with Ca F232.52%,fluorite concentrate with Ca F297.55%, Si O21.36%, the recovery of fluorite was 76.67%; under the pulp temperature of 10℃, the industrial indicators were as follows:fluorite ore with Ca F230.14%,fluorite concentrate with Ca F296.37%,Si O21.81%,the recovery of fluorite was 71.67%.