Investigation on three-dimensional localization characteristics of Fluorescence self-interference digital holography

We proposed here a new method of encoding and extracting the 3D position of fluorescent micro particles. Cross-correlation matching method is employed to localize the axial position of fluorescent microsphere. And the localization accuracy of the proposal is compared with that of the traditional diffraction reconstruction algorithm. The effectiveness of the method is verified by both simulations and experiments, and the localization accuracy of the method are evaluated quantitatively. This method overcomes the limitation of the imaging depth in the traditional single molecule localization, reduces the influence of optical aberration on the localization accuracy, and it possess the potential of achieving the isotropic super resolution localization of the sample.