Research Article Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Lamivudine, Zidovudine and Nevirapine from Bulk and Tablet Dosage Form

A simple, fast and precise reverse phase high performance liquid chromatographic method developed for the simultaneous determination of Lamivudine, Zidovudine & Nevirapine in its tablets form. Nucleodur C18 250 x 4.6 mm(l x d),5µ in isocratic mode with mobile phase Buffer and Methanol in the ratio of 60 : 40 %v/v were used. The flow rate was 0.8ml/min. Linearity for Lamivudine, Zidovudine & Nevirapine were 24mcg/ml to 36mcg/ml,48mcg/ml to 72mcg/ml and 32mcg/ml to 48mcg/ml respectively. The correlation coefficient (r 2 ) was found to be greater than (0.999). Amount of Lamivudine, Zidovudine & Nevirapine present in each tablet were found to be 150.20mg/tab and 300.07mg/tab and 199.52mg/tab respectively. The %RSD values were less than 2% for method precision. The mean percentage recovery of Lamivudine, Zidovudine & Nevirapine were found to be 100.313, 100.79, & 99.96.respectively.The proposed method is accurate, precise, selective and rapid for the simultaneous estimation of Lamivudine, Zidovudine & Nevirapine.