In Vivo Chemistry for Pretargeted Tumor Imaging in Live Mice

Mice and men: An antibody conjugate with trans-cyclooctene was administered to tumor-bearing mice, and the resulting chemically tagged tumors were subsequently treated with an 111In-labeled tetrazine probe in an inverse-electron-demand Diels–Alder reaction. The adduct was formed in a remarkable 52–57 % yield in vivo and used for non-invasive pretargeted tumor imaging in mice (see picture). Detailed facts of importance to specialist readers are published as "Supporting Information". Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.