造血
转录因子
细胞生物学
生物
突变体
祖细胞
细胞命运测定
免疫系统
抄写(语言学)
转导(生物物理学)
巨噬细胞
细胞分化
分子生物学
干细胞
基因
遗传学
生物化学
体外
哲学
语言学
作者
Rodney P. DeKoter,Harinder Singh
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2000-05-26
卷期号:288 (5470): 1439-1441
被引量:660
标识
DOI:10.1126/science.288.5470.1439
摘要
The ets family transcription factor PU.1 is required for the development of multiple lineages of the immune system. Using retroviral transduction of PU.1 complementary DNA into mutant hematopoietic progenitors, we demonstrate that differing concentrations of the protein regulate the development of B lymphocytes as compared with macrophages. A low concentration of PU. 1 protein induces the B cell fate, whereas a high concentration promotes macrophage differentiation and blocks B cell development. Conversely, a transcriptionally weakened mutant protein preferentially induces B cell generation. Our results suggest that graded expression of a transcription factor can be used to specify distinct cell fates in the hematopoietic system.
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