CTL公司*
表位
生物
病毒学
细胞毒性T细胞
外周血单个核细胞
体外
肽
人类白细胞抗原
抗原
分子生物学
免疫学
生物化学
作者
Hee Gu Lee,Jong‐Seok Lim,Ki-Young Lee,Yong K. Choi,In-Seong Choe,Tai-Wha Chung,Kilhyoun Kim
出处
期刊:Virus Research
[Elsevier]
日期:1997-08-01
卷期号:50 (2): 185-194
被引量:23
标识
DOI:10.1016/s0168-1702(97)00068-3
摘要
Cytotoxic T lymphocytes (CTL) recognize and destroy virus-infected cells, and it has been established that epitope-based peptides could induce such CTL in vivo as well as in vitro. In this study attempts were made to define the epitopes that are recognized by the CTL, and thus a series of 9- to 10-mer peptides derived from the amino acid sequences of hepatitis B virus (HBV) proteins were synthesized on the basis of the previously described HLA-A2 peptide binding motif. The binding assay of the synthetic peptides using transporter-associated with antigen processing (TAP)-deficient human cell line, T2, showed that eight out of 11 peptides tested enhanced the expression of HLA-A2 molecules on the T2 cell surface. Some of these peptides triggered activation of CTL in peripheral blood mononuclear cells of HBV-seropositive chronic carriers. The activated CTL in turn recognized and killed the T2 cells pulsed with the same peptides. This study shows that novel HLA-A2-restricted epitopes exist in the natural repertoire of immunity against HBV. These findings can be useful in developing peptide-based therapeutics against viral infections.
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