替加环素
肺炎克雷伯菌
生物
脉冲场凝胶电泳
质粒
微生物学
产气肠杆菌
背景(考古学)
拉伤
抗生素耐药性
遗传学
抗菌剂
基因
基因型
大肠杆菌
抗生素
解剖
古生物学
作者
Xiaojuan Wang,Henan Li,Chunjiang Zhao,Hongbin Chen,Jingbo Liu,Zhanwei Wang,Wang Qi,Yawei Zhang,Wenqiang He,Feifei Zhang,Hui Wang
标识
DOI:10.1016/j.ijantimicag.2014.04.010
摘要
The aim of this study was to characterise a tigecycline-resistant blaNDM-9-bearing Klebsiella aerogenes strain (HNHF1) of chicken origin.Strain HNHF1 was characterised by phenotypic antimicrobial susceptibility testing, PCR, conjugation assays, S1 nuclease pulsed-field gel electrophoresis (S1-PFGE), whole-genome sequencing and bioinformatics analysis.The blaNDM-9 gene was located on an IncHI2 plasmid (pHNHF1_NDM-9) carrying various antimicrobial resistance genes. Moreover, the genetic context ΔISAba125–blaNDM-9–bleMBL–trpF is similar to other blaNDM-bearing genetic contexts. TA cloning experiments showed that tet(A) variants may play a partial role in high-level tigecycline resistance in HNHF1.This is the first report of a tigecycline-resistant blaNDM-9-bearing IncHI2 plasmid in a K. aerogenes ST4 isolate of animal origin, which poses a great threat to public health. Further comprehensive surveillance is needed.
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