胃蛋白酶
超声波
萃取(化学)
超声
化学
色谱法
Ⅰ型胶原
十二烷基硫酸钠
琼脂糖凝胶电泳
凝胶电泳
生物化学
生物
酶
医学
内分泌学
基因
放射科
作者
Jianxing He,Rui Shi,Rimutu Ji
出处
期刊:Polymers
[MDPI AG]
日期:2023-04-19
卷期号:15 (8): 1943-1943
被引量:2
标识
DOI:10.3390/polym15081943
摘要
The objective of this study was to evaluate the effect of ultrasound pre-treatment on the characterization from Bactrian camel skin. It was possible to produce and characterize collagen extracted from Bactrian camel skin. The results showed that the yield of collagen was higher in ultrasound pre-treatment (UPSC) (41.99%) than the pepsin-soluble collagen extraction (PSC) (26.08%). All extracts were identified as type I collagens using sodium dodecyl sulfate polyacrylamide gel electrophoresis and retained their helical structure, as confirmed through Fourier transform infrared spectroscopy. The scanning electron microscopy analysis of UPSC revealed that some physical changes were caused by sonication. UPSC had smaller particle size than PSC. The viscosity of UPSC always plays a leading role in the range of 0-10 Hz. However, the contribution of elasticity to the solution system of PSC increased in the range of 1-10 Hz. Moreover, ultrasound-treated collagen had superior solubility property at pH 1-4 and at <3% (w/v) NaCl than non-ultrasound treated collagen. Therefore, the utilization of ultrasound for the extraction of pepsin soluble collagen is a good alternative technology to expand the application at industrial level.
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