Optimization of a gelatin/carboxymethylcellulose‐based probiotic microcapsule and its application in preventing dextran sodium sulfate–induced colitis in mice

Abstract Oral administration of probiotics has demonstrated substantial potential in alleviating colitis. However, most of the ingested microorganisms struggle to survive the harsh conditions of the gastrointestinal tract, leading to decreased efficacy. In the present study, using double emulsification (W 1 /O/W 2 ) and complex coacervation methods, we developed a gelatin/carboxymethyl cellulose (CMC)‐based probiotic microcapsule and analyzed the efficacy of encapsulated probiotics in preventing dextran sodium sulfate (DSS)‐induced colitis in mice. Our results reveal that nearly 90% of the encapsulated probiotics remained viable after 30‐day storage at 4°C and approximately 38.1% of viable bacteria (4.0 × 10 8 cfu/g) survived after 4‐h simulated gastrointestinal digestion. In a DSS‐induced colitis model, pretreatment with probiotics exerted significant protective effects, with the bacterial microcapsule‐treated group having superior outcomes to those of the bacterial suspension plus empty carrier group. Probiotic treatments, especially those administered in the encapsulated form, significantly increased fecal short‐chain fatty acid contents, and altered the intestinal microbial composition. The family Muribaculaceae , dominant bacteria in the mouse gut, may be the key microorganism involved in the BM regulation process. Our study presents an alternative approach to treating colitis using probiotics. Practical Application The encapsuled probiotic showed remarkable storage stability at 4°C, maintained good vitality after simulated digestion, and gained superior outcomes in preventing colitis. Our results offer an alternative approach for the probiotic preparations aiming to prevent the intestinal inflammation.