生物结合
荧光蛋白
活体细胞成像
超分辨率
荧光
纳米技术
绿色荧光蛋白
光学成像
蛋白质标签
荧光寿命成像显微镜
化学
计算生物学
细胞
计算机科学
生物
生物化学
人工智能
材料科学
重组DNA
融合蛋白
基因
物理
量子力学
光学
图像(数学)
作者
Dilizhatai Saimi,Zhixing Chen
摘要
Abstract Imaging proteins with high resolution is crucial for studying cellular physiology and pathology. Fluorescence imaging is a privileged method to visualize proteins with subcellular precision in live cells. In recent years, there has been a tremendous advance in the field of fluorescent dyes that are optically more sophisticated than genetically‐encodable fluorescent proteins. In this review, we aim to discuss modern bioconjugation methods to specifically incorporate these dyes into protein‐of‐interests. We focus on advances in live‐cell labeling strategies and fluorescent probes, especially the HaloTag, SNAP‐tag, TMP‐tag, and unnatural amino acid systems and their applications. These protein labeling methods, along with cutting‐edge dyes and novel microscopy methods, have become the infrastructure for biological research in the era of super‐resolution imaging.
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