Dry vacuum‐assisted hydration method: A new accelerating method for reconstitution of injectable Poly‐D,L‐lactic acid

体积热力学 注射器 化学 柱塞 材料科学 色谱法 复合材料 机械工程 物理 工程类 量子力学
作者
Jui‐Yu Lin,Chuan‐Yuan Lin,Victor Mun‐Khan Cheong
出处
期刊:Journal of Cosmetic Dermatology [Wiley]
卷期号:23 (4): 1489-1491 被引量:1
标识
DOI:10.1111/jocd.16097
摘要

Injectable poly-D,L-lactic acid (PDLLA) is a collagen-stimulating filler (AestheFill; REGEN) supplied as lumps of lyophilized powder in vials. Reconstitution with sterile water for injection (SWFI) is required before injection.1, 2 Typically, this involves a reconstitution time of 20–30 min by adding SWFI into the vial and shaking. In 2020, Lin et al.3, 4 proposed a rapid “back-and-forth” reconstitution method, which takes about 5–10 min. Considering that the hydration time of the PDLLA lumps is a crucial step in the reconstitution process, in 2021, they introduced an even faster “vacuum-assisted hydration” (VAH) prior to the back-and-forth technique, which only takes less than 1 min.5 In 2023, Victor Cheong modified and proposed a different VAH method. This article aims to introduce this new method and compare it with the previous VAH method. Lin's VAH method relies on the Boyle's law, which establishes a reciprocal correlation between the pressure and volume of a constant quantity of gas.5, 6 This method is described as follows: A P-syringe housing PDLLA lumps is securely attached to an S-syringe containing SWFI via a 3-way stopcock. After pushing the SWFI into the P-syringe and expelling most of the air, the plunger of the P-syringe is pulled to create negative pressure. At this point, the air within the PDLLA lumps expands, bursts out, and rises to the water surface. Upon releasing the plunger, the air volume within the lumps immediately contracts, drawing surrounding SWFI into them swiftly. The hydration process for all the PDLLA lumps can be completed by repeating these steps twice.5 (Figure 1; Video 1) Cheong's VAH method also relies on the Boyle's law. A P-syringe housing PDLLA lumps and an unoccupied S-syringe are firmly linked through a 3-way stopcock. Then, the plunger of the S-syringe is withdrawn to introduce a negative pressure within both syringes. After that, the valve of 3-way stopcock is turned to close the opening of the P-syringe to maintain the negative pressure inside. By repeating these steps twice, the pressure inside the P-syringe is near a vacuum. Next, draw the desired amount of water into the S-syringe. Reconnect it to the 3-way stopcock. Upon opening the valve connecting these two syringes, the SWFI will be drawn quickly into the P-syringe along with the PDLLA lumps, facilitated by the existing vacuum. The hydration process of all the PDLLA lumps is also very short using this method. (Figure 2; Video 2). To distinguish between these two methods, we refer to Lin's method as the “wet” VAH method, while Cheong's method is known as the “dry” VAH method. Both methods are grounded in Boyle's law, capitalizing on the rapid air volume/pressure changes within PDLLA lumps to expedite the hydration process. The key distinction lies in the vacuum procedure, where it occurs before or after soaking PDLLA lumps in SWFI. In the hands of experienced practitioners, both wet and dry VAH methods are straightforward and innovative, often taking no more than 1 min to complete. However, there are certain drawbacks associated with these methods. The transfer of PDLLA lumps from the vial to the P-syringe may pose a risk of contamination or accidental spillage.4 Moreover, practitioners must possess a thorough understanding of the entire procedure, as any air leakage during the creation of vacuum within the P-syringe could lead to process failure. In conclusion, both wet and dry VAH methods offer simplicity and speed. Nonetheless, strict adherence to aseptic procedures is paramount, and practitioners must have a comprehensive grasp of the entire process. The authors specially thank Sunny Fu and Wen-Yi Yen for their kindly assistance for the video clip filming and editing. The wording of this manuscript is modified by ChatGPT-3.5. Dr. Lin J-Y and Dr. Lin C-Y are medical consultants of Jiangsu Wuzhong Aesthetic Biotech., and medical directors of REGEN Biotech. Dr. Cheong V M-K is a speaker for REGEN Biotech. No ethical approval was required as this artical has no original research data. Video 1. Video 2. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
lq8996发布了新的文献求助10
2秒前
ouwenwen发布了新的文献求助10
2秒前
丘比特应助过雨露采纳,获得10
5秒前
6秒前
6秒前
jingyi发布了新的文献求助10
10秒前
10秒前
科研通AI5应助JoyChu采纳,获得10
13秒前
000发布了新的文献求助10
13秒前
13秒前
jenningseastera应助Bin_Liu采纳,获得10
15秒前
不可发布了新的文献求助10
15秒前
15秒前
16秒前
饼子完成签到,获得积分10
18秒前
木木发布了新的文献求助20
19秒前
19秒前
无限寻雪发布了新的文献求助30
20秒前
zz完成签到,获得积分10
20秒前
WDS应助菜狗采纳,获得20
20秒前
饼子发布了新的文献求助10
20秒前
科研通AI5应助坚定的语芙采纳,获得10
21秒前
21秒前
zzz完成签到,获得积分10
22秒前
深情安青应助jingyi采纳,获得10
23秒前
Orange应助黄小静采纳,获得10
23秒前
25秒前
彭于晏应助东东采纳,获得30
31秒前
无花果应助大侦探皮卡丘采纳,获得10
33秒前
36秒前
hh发布了新的文献求助10
36秒前
36秒前
Jasper应助苏轼他小姨采纳,获得10
36秒前
zho应助less12323采纳,获得10
36秒前
38秒前
沉默的钻石完成签到,获得积分10
38秒前
39秒前
花成花发布了新的文献求助10
41秒前
大模型应助不要讨好十三采纳,获得10
41秒前
42秒前
高分求助中
Mass producing individuality 600
Разработка метода ускоренного контроля качества электрохромных устройств 500
A Combined Chronic Toxicity and Carcinogenicity Study of ε-Polylysine in the Rat 400
Lab Dog: What Global Science Owes American Beagles 200
Governing Marine Living Resources in the Polar Regions 200
Bazaar to piazza. Islamic trade and Italian art, 1300–1600 200
Encyclopaedia Britannica 200
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 物理 生物化学 纳米技术 计算机科学 化学工程 内科学 复合材料 物理化学 电极 遗传学 量子力学 基因 冶金 催化作用
热门帖子
关注 科研通微信公众号,转发送积分 3824428
求助须知:如何正确求助?哪些是违规求助? 3366731
关于积分的说明 10442366
捐赠科研通 3086057
什么是DOI,文献DOI怎么找? 1697679
邀请新用户注册赠送积分活动 816458
科研通“疑难数据库(出版商)”最低求助积分说明 769676