苹果酸
生物
苹果属植物
基因
遗传学
种质资源
基因座(遗传学)
基因表达
人口
植物
生物化学
柠檬酸
人口学
社会学
作者
Litong Zheng,Liao Liao,Chenbo Duan,Wenfang Ma,Yunjing Peng,Yangyang Yuan,Yuepeng Han,Fengwang Ma,Mingjun Li,Baiquan Ma
出处
期刊:Plant Physiology
[Oxford University Press]
日期:2023-02-22
卷期号:192 (3): 1877-1891
被引量:4
标识
DOI:10.1093/plphys/kiad111
摘要
Acidity is a key determinant of fruit organoleptic quality. Here, a candidate gene for fruit acidity, designated MdMYB123, was identified from a comparative transcriptome study of two Ma1Ma1 apple (Malus domestica) varieties, "Qinguan (QG)" and "Honeycrisp (HC)" with different malic acid content. Sequence analysis identified an A→T SNP, which was located in the last exon, resulting in a truncating mutation, designated mdmyb123. This SNP was significantly associated with fruit malic acid content, accounting for 9.5% of the observed phenotypic variation in apple germplasm. Differential MdMYB123- and mdmyb123-mediated regulation of malic acid accumulation was observed in transgenic apple calli, fruits, and plantlets. Two genes, MdMa1 and MdMa11, were up- and down-regulated in transgenic apple plantlets overexpressing MdMYB123 and mdmyb123, respectively. MdMYB123 could directly bind to the promoter of MdMa1 and MdMa11, and induce their expression. In contrast, mdmyb123 could directly bind to the promoters of MdMa1 and MdMa11, but with no transcriptional activation of both genes. In addition, gene expression analysis in 20 different apple genotypes based on SNP locus from "QG" × "HC" hybrid population confirmed a correlation between A/T SNP with expression levels of MdMa1 and MdMa11. Our finding provides valuable functional validation of MdMYB123 and its role in the transcriptional regulation of both MdMa1 and MdMa11, and apple fruit malic acid accumulation.
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