脂肪酶
生物柴油
毕赤酵母
水解物
甘油
中心组合设计
Plackett–伯曼设计
藻类生质燃料
食品科学
生物柴油生产
生物量(生态学)
化学
木质纤维素生物量
色谱法
植物
生物
响应面法
发酵
生物化学
重组DNA
水解
酶
农学
催化作用
基因
作者
J. Iyyappan,J. Jayamuthunagai,B. Bharathiraja,A Saravanaraj,Rajesh Kumar,S. Balraj
标识
DOI:10.1016/j.biortech.2022.127893
摘要
In this study, Caulerpa racemosa oil was used to produce biodiesel by recombinant Pichia pastoris displaying bound (rPp-BL) and secretory lipase (rPp-SL). Collected algae was pre-treated using ultrasonication, microwave and solvent extraction. Defatted C. racemosa was subjected to dilute acid treatment to obtain algal biomass hydrolysate. Both rPp-BL and rPp-SL were cultivated in algal biomass hydrolysate and glycerol. Surfactant treatment was performed on rPp-BL. Screening and optimization of variables were performed for biodiesel production using Plackett Burman design and central composite design, respectively. About 10.64 % (w/w) of algal oil was extracted from C. racemosa. Both rPp-BL and rPp-SL effectively utilized C. racemosa biomass hydrolysate and glycerol. rPp-SL combined with triton X (1.0 % w/v) treated rPp-BL for 3 min improved lipase activity. Methanol to oil ratio, combined whole cell biocatalyst and temperature were significant factors. Under optimum conditions, biodiesel yield reached about 93.64 % after 30 h using developed whole cell biocatalyst.
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