脂质微区
脂筏
内吞作用
膜
分区(防火)
细胞生物学
生物膜
膜脂
生物
木筏
化学
细胞
生物化学
酶
有机化学
共聚物
聚合物
作者
Martin-Paul Agbaga,Mark E. McClellan,Michael H. Elliott
出处
期刊:Methods in molecular biology
日期:2023-01-01
卷期号:: 129-139
标识
DOI:10.1007/978-1-0716-2966-6_12
摘要
The original concept that lipid and protein components are randomly distributed in cellular membranes has been challenged by evidence of compartmentalization of such components into discrete membrane microdomains (known as lipid rafts). The lipid microdomain hypothesis has generated significant controversy and rigorous inquiry to test the idea that such domains concentrate machinery to mediate cellular processes such as signaling, synaptic plasticity, and endocytosis. As such, a large number of studies have used biochemical, cell biological, and biophysical methodologies to define the composition of membrane microdomains in experimental contexts. Although biochemical preparation strategies are not without limitations (as discussed herein), the isolation of detergent-resistant and detergent-free membrane domains can provide important information about the segregation of lipids and proteins in membranes. In this chapter, we describe methodologies to isolate membranes from cell or tissue sources with biophysical/biochemical properties of membrane microdomains and also provide methods for subsequent classical or mass spectrometry-based lipid analytical approaches.
科研通智能强力驱动
Strongly Powered by AbleSci AI