化学
藤黄属
NF-κB
下调和上调
药理学
炎症
活力测定
肿瘤坏死因子α
消炎药
信号转导
促炎细胞因子
生物化学
传统医学
生物
细胞凋亡
医学
免疫学
基因
作者
Pathanin Chantree,Pongsakorn Martviset,Nattaya Thongsepee,Kant Sangpairoj,Phornphan Sornchuer
出处
期刊:Nutrients
[MDPI AG]
日期:2023-01-22
卷期号:15 (3): 575-575
摘要
Garcinia is a significant medicinal plant with many beneficial phytoconstituents, including garcinol. This study investigated the anti-inflammatory effect of garcinol isolated from Garcinia dulcis fruit in LPS-activated THP-1 and Raw 264.7 macrophages. The results demonstrated that the low concentration of garcinol did not alter cell viability. Furthermore, co-incubation of garcinol with LPS inhibited the production of pro-inflammatory cytokines, including TNF-α, IL-8, IL-6, IL-1β, and pro-inflammatory mediators, including iNOS and COX-2 at the mRNA and protein expression levels. Garcinol also decreased the secretion of TNF-α, IL-6, IL-1β, PGE2, and NO. Moreover, the anti-inflammatory effects involved an alteration in the NF-κB signaling pathway. Downregulation of pIKKα/β, pIκBα, and pNF-κB was observed, hence reducing the translocation of pNF-κB from the cytosol into the nucleus, which subsequently decreased the production of pro-inflammatory molecules. Therefore, garcinol isolated from Garcinia dulcis is a potential candidate as an anti-inflammatory agent for inflammation-related disease treatment.
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