This paper deals with the problem of state of health (SOH) estimation of lithium-ion battery. In order to validate the performance of lithium-ion battery, an accelerated aging experiment of the battery is designed. Based on the interval capacity corresponding to the voltage range from 3.95 V to 4.15 V, the least square method is introduced to estimate the battery SOH. Finally, a battery test bench is established, and the effectiveness of the proposed method is verified via simulation and experiment.