EdU Immunohistochemistry: Chromogen detection of thaw-mounted sections v1

EdU (5-ethynyl-2′-deoxyuridine) is a high-speed, reportedly-safe method for detecting proliferating cells by incorporating a thymidine analog into newly synthesized DNA during S phase of mitosis. EdU detection utilizes "click chemistry" for detection, eliminating the need for DNA denaturation and better preserving cellular morphology and antigens when compared to BrdU antigen retrieval and labeling. This protocol utilizes the Invitrogen Click-IT kit for EdU Cell Proliferation Kit and can be multiplexed with additional lineage or state antibodies as desired.