Inductive Coculture Differentiation of Induced Pluripotent Stem Cells into Cardiomyocytes

Various types of stem cells and nonstem cells have been shown to differentiate or transdifferentiate into cardiomyocytes by way of coculture with appropriate inducer cells. Here we describe a method to induce cardiac differentiation in induced pluripotent stem (iPS) cells through the use of coculture with previously differentiated iPS cell-derived cardiomyocytes (iCMs). This differentiation process can be achieved without the use of exogenous pathway inhibitors and morphogens.