多菌灵
蜂王浆
生物
孵卵
蜜蜂
杀菌剂
杀虫剂
毒理
细胞生物学
植物
生态学
作者
Kang Wang,Heng Chen,Zheguang Lin,Qingsheng Niu,Zhi Wang,Fuchao Gao,Ting Ji
出处
期刊:Chemosphere
[Elsevier]
日期:2021-03-01
卷期号:266: 129011-129011
被引量:13
标识
DOI:10.1016/j.chemosphere.2020.129011
摘要
Studying the sublethal effects of agrochemical pesticides on nontarget honeybees (Apis mellifera) is important for agricultural development. Carbendazim is a widely used broad-spectrum fungicide that inhibits mitotic microtubule formation and cell division. However, the impact of carbendazim on bee health and development has not been fully elucidated. Here, using proteomics approaches, we assessed in vitro the changes in the expression of functional proteins in the head of newly emerged adults following treatment with field concentration of carbendazim during the larval stage. Treatment with carbendazim severely altered 266 protein expression patterns in the heads of adults and 218 of them showed downregulation after carbendazim exposure. Notably, major royal jelly proteins, a crucial multifunctional protein family with irreplaceable function in sustaining the development of colonies, were significantly suppressed in carbendazim-treated bees. This result was verified in both head and hypopharyngeal gland of nurse bees. Moreover, visual and olfactory loss, immune functions, muscular activity, social behavior, neural and brain development, protein synthesis and modification, and metabolism-related proteins were likely inhibited by carbendazim treatment. Together, these results suggest that carbendazim is an environmental risk factor that likely weakens bee colonies, partially due to reduced expression of major royal jelly proteins, which may be potential causes of colony collapse disorder.
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