Characterization and mechanism of Mn(II)-based mixotrophic denitrifying bacterium (Cupriavidus sp. HY129) in remediation of nitrate (NO3--N) and manganese (Mn(II)) contaminated groundwater.

Abstract The co-contamination of groundwater with nitrate (NO3−-N) and manganese (Mn(II)) is a global issue that needs to be efficiently remediated. In this research, a novel denitrifying and manganese-oxidizing strain HY129 was isolated from the sediments sample of a drinking water and identified as Cupriavidus sp. HY129. The remediation ability of strain HY129 regarding the nitrate and Mn(II) pollution were investigated. The removal efficiency of nitrate and Mn(II) were 99.81% (0.229 mgL−1 h−1) and 87.24% (0.233 mgL−1 h−1) in bacterial culture after 72 h, respectively. Moreover, the addition of Mn(II) significantly enhanced the denitrification process, while excessive concentration of Mn(II) caused more NO2−-N accumulation. The impacts of adsorption and oxidation activity on Mn(II) removal were investigated. Protein in extracellular polymeric substance (EPS) which produced in the Mn-oxidizing process was speculated to be the main cause of extracellular adsorption of Mn(II). Characterization of biogenic manganese oxides (BMO) confirmed the formation of high-valent manganese and the trapping experiment with sodium pyrophosphate (NaPP) demonstrated the existence of Mn(III)-intermediates. Furthermore, multicopper oxidase gene amplification provided evidence for the molecular biology of Mn(II) oxidation by strain HY129.