缝隙连接
连接蛋白
细胞生物学
细胞内
细胞外
分泌物
小泡
胞外囊泡
微泡
胞吐
细胞外小泡
生物
缺血
内科学
生物化学
医学
膜
基因
小RNA
作者
Tânia Martins‐Marques,Teresa Ribeiro‐Rodrigues,Saskia C.A. de Jager,Mónica Zuzarte,Cátia Ferreira,Pedro F. Cruz,Liliana Reis,Rui Baptista,Lino Gonçalves,Joost P.G. Sluijter,Henrique Girão
出处
期刊:Life science alliance
[Life Science Alliance]
日期:2020-10-23
卷期号:3 (12): e202000821-e202000821
被引量:27
标识
DOI:10.26508/lsa.202000821
摘要
Ischemic heart disease has been associated with an impairment on intercellular communication mediated by both gap junctions and extracellular vesicles. We have previously shown that connexin 43 (Cx43), the main ventricular gap junction protein, assembles into channels at the extracellular vesicle surface, mediating the release of vesicle content into target cells. Here, using a comprehensive strategy that included cell-based approaches, animal models and human patients, we demonstrate that myocardial ischemia impairs the secretion of Cx43 into circulating, intracardiac and cardiomyocyte-derived vesicles. In addition, we show that ubiquitin signals Cx43 release in basal conditions but appears to be dispensable during ischemia, suggesting an interplay between ischemia-induced Cx43 degradation and secretion. Overall, this study constitutes a step forward for the characterization of the signals and molecular players underlying vesicle protein sorting, with strong implications on long-range intercellular communication, paving the way towards the development of innovative diagnostic and therapeutic strategies for cardiovascular disorders.
科研通智能强力驱动
Strongly Powered by AbleSci AI