Abstract The aquatic organisms, human beings and other living species are affected due to polluted water excreted from industries. This polluted water contains various chemical substances such as dyes, cyanide etc. One such compound is Methylene blue dye which has an adverse effect on many living organisms. In this study Titanium dioxide (TiO 2 ) nanoparticles was prepared which completely neutralize the effect of Methylene blue in water. The morphology of titanium dioxide has been studied through Scanning Electron Microscope (SEM). The efficiency of prepared nanoparticles has been tested in the presence of sunlight and aeration. The efficiency of Titanium dioxide was 85% at the end of 15 minutes which prohibit the effect of Methylene blue.