神经炎症
小胶质细胞
电池类型
生物
细胞
冲程(发动机)
基因
下调和上调
疾病
转录组
神经科学
炎症
基因表达
细胞生物学
医学
病理
免疫学
遗传学
工程类
机械工程
作者
Kai Zheng,Lingmin Lin,Wei Jiang,Lin Chen,Xiyue Zhang,Qian Zhang,Yi Ren,Junwei Hao
标识
DOI:10.1177/0271678x211026770
摘要
Ischemic stroke (IS) is a detrimental neurological disease with limited treatments options. It has been challenging to define the roles of brain cell subsets in IS onset and progression due to cellular heterogeneity in the CNS. Here, we employed single-cell RNA sequencing (scRNA-seq) to comprehensively map the cell populations in the mouse model of MCAO (middle cerebral artery occlusion). We identified 17 principal brain clusters with cell-type specific gene expression patterns as well as specific cell subpopulations and their functions in various pathways. The CNS inflammation triggered upregulation of key cell type-specific genes unpublished before. Notably, microglia displayed a cell differentiation diversity after stroke among its five distinct subtypes. Importantly, we found the potential trajectory branches of the monocytes/macrophage's subsets. Finally, we also identified distinct subclusters among brain vasculature cells, ependymal cells and other glia cells. Overall, scRNA-seq revealed the precise transcriptional changes during neuroinflammation at the single-cell level, opening up a new field for exploration of the disease mechanisms and drug discovery in stroke based on the cell-subtype specific molecules.
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