生物信息学
化学
抑制性突触后电位
生物化学
体外
姜黄素
二肽基肽酶
消化(炼金术)
胃抑制多肽
肽
高粱
对接(动物)
寡肽
酶
生物
色谱法
医学
基因
护理部
神经科学
激素
生态学
胰高血糖素
作者
Lingyan Dai,Lingxin Kong,Xia Cai,Peng Jiang,Nian Liu,Dongjie Zhang,Zhijiang Li
标识
DOI:10.1021/acs.jafc.1c04484
摘要
Potential dipeptidyl peptidase IV (DPP-IV) inhibitory oligopeptides from sorghum kafirin were developed using in silico and in vitro methodologies for the management of diabetes. Twenty-eight peptides with 5-10 residues were identified from the papain hydrolysates of sorghum kafirin. Sixteen nontoxic DPP-IV inhibitory peptides were screened with a computer method based on molecular docking. Molecular docking revealed that LPFYPQ (LP6), GPVTPPILG (GP9), and LPFYPQGV (LP8) effectively inactivated DPP-IV by binding to its active sites with a low interaction energy. An in silico analysis of these three inhibitory oligopeptides indicated that they were all bound to the S1 and S2 active pockets of DPP-IV through hydrogen bonds and hydrophobic interactions. The in vitro inhibitory activity was also verified. The DPP-IV inhibitory activities of LP6 and LP8 decreased after gastric digestion and remained stable after intestinal digestion, and the GP9 inhibitory activity remained stable after gastrointestinal digestion. Experimental results from Caco-2 cells showed further inhibitory effects of oligopeptides on DPP-IV. The results are relevant to the exploration of biofunctional DPP-IV inhibitory peptides from sorghum as a treatment for patients with diabetes or in medical research.
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