Preparation and characterization of sodium alginate–PVA polymeric scaffolds by electrospinning method for skin tissue engineering applications

Sodium alginate (SA) has proven its high potential in tissue engineering and regenerative medicine. One of the main weaknesses of this polysaccharide is its low spinnability. Nanofiber-based scaffolds are of interest to scientists for biomedical engineering. The main aim of this study was to improve the spinnability of SA in combination with polyvinyl alcohol (PVA). The main parameters in the electrospinning of the optimized SA:PVA ratio, including voltage, flow rate, and working space were also optimized. To achieve this, response surface methodology under central composite design was employed to design the experiments scientifically. The final nanofiber scaffolds were studied using scanning electron microscopy, Fourier transform infrared spectroscopy for degradability, swelling, tensile strength, porosity, nanofiber diameter, contact angle, and cytotoxicity. Based on the results, the best ratio for SA : PVA was 1 : 6.5 that was spinnable in various values for the process parameters. The fabricated scaffolds under these conditions revealed good physical, chemical, mechanical, and biological features. L929 cell lines revealed high viability during 48 h culture. The results revealed that uniform and homogeneous nanofibers with regular size distribution (166 nm) were obtained at 30 kV, 0.55 μL h-1, and 12.50 cm. To sum up, the fabricated scaffolds with the optimized ratio under the reported conditions indicate at good biologically compatible candidates for skin tissue engineering.