生物
蛭弧菌
生物膜
金黄色葡萄球菌
微生物学
捕食
细菌
捕食者
革兰氏阴性菌
克
生态学
大肠杆菌
遗传学
基因
作者
Hansol Im,Mohammed Dwidar,Robert J. Mitchell
出处
期刊:The ISME Journal
[Springer Nature]
日期:2018-05-30
卷期号:12 (8): 2090-2095
被引量:41
标识
DOI:10.1038/s41396-018-0154-5
摘要
Bdellovibrio bacteriovorus HD100 is a predatory bacterium which lives by invading the periplasm of Gram-negative bacteria and consuming them from within. Although B. bacteriovorus HD100 attacks only Gram-negative bacterial strains, our work here shows attack-phase predatory cells also benefit from interacting with Gram-positive biofilms. Using Staphylococcus aureus biofilms, we show this predator degrades the biofilm matrix, obtains nutrients and uses these to produce and secrete proteolytic enzymes to continue this process. When exposed to S. aureus biofilms, the transcriptome of B. bacteriovorus HD100 was analogous to that seen when present intraperiplasmically, suggesting it is responding similarly as when in a prey. Moreover, two of the induced proteases (Bd2269 and Bd2692) were purified and their activities against S. aureus biofilms verified. In addition, B. bacteriovorus HD100 gained several clear benefits from its interactions with S. aureus biofilms, including increased ATP pools and improved downstream predatory activities when provided prey.
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