化学
半色移
喹啉
荧光
质子化
水溶液
分析化学(期刊)
光化学
质子核磁共振
共振(粒子物理)
色谱法
立体化学
有机化学
粒子物理学
物理
离子
量子力学
作者
Zhen Liu,Guoping Li,Yana Wang,Jiulong Li,Yang Mi,Dapeng Zou,Tiesheng Li,Yangjie Wu
出处
期刊:Talanta
[Elsevier]
日期:2019-01-01
卷期号:192: 6-13
被引量:38
标识
DOI:10.1016/j.talanta.2018.09.026
摘要
Herein, a novel quinoline-based fluorescent probe DQPH has been developed for ratiometric detection of subtle pH fluctuation in biosystem. Upon altering the pH from 4.50 to 9.00, the emission spectra exhibit a large hypsochromic shift (57 nm) and the ratio of fluorescence intensity (F531 nm/F588 nm) changes from 0.30 to 1.36 with an ideal pKa value of 7.18 and a linear pH variation range of 6.35-8.00. The ratiometric response is attributed to the protonation-activable resonance charge transfer (PARCT) process, which has been proved by 1H NMR and NOESY experiment. This probe displayed good solubility, low cytotoxicity, anti-interference capability and reversible pH sensing. Furthermore, DQPH was successfully applied for monitoring pH changes in living cells.
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