The paper study synthesis,structural characterization of rutin complexes with Cu(Ⅱ)、Zn(Ⅱ)、Mn(Ⅱ).The result is that the complexes may be formulated as [M3L2(H2O)n](CH3COO)2(n=1,2,3…) and ligand three hydroxy groups on the unsaturated rings take parts in the coordination with metal ions.Determinated the Moore conductivity and IR by CV,the antibacterial activity is of significantion.