[Objective] The aim of the research was to increase the yield of potato resistant starch.[Method] With potato starch as materials and the yield of resistant starch as evaluation index,the optimum preparation conditions for potato resistant starch were obtained through orthogonal test.[Result] The optimum preparation conditions for potato resistant starch were as follows: starch milk concentration was 30 %,pH value of starch milk was 6,hot-press temperature was 120 ℃,autoclaving time was 40 min and the yield of resistant starch was 9.77 %.[Conclusion] The times of heating-cooling treatment had greater effects on the formation of resistant starch.With the increasing of times,the quantity of the formed resistant starch was also increased.