Molecular analysis of bacterial diversity with the two lakes in Xinjiang

The bacterial diversity of Xiaokuer Lake and Tuzi Lake located in Xinjiang was investigated through a culture-independent approach by constructing 16S rDNA clone libraries whose 16S rDNA was proliferated by PCR using bacterial universal primers.For the bacterial rDNA clone libraries,32 clones randomly selected in each lake was sequenced for phylogenetic analysis.The comparative analysis of bacterial diversity on the two lakes was made as well by several diversity index including Shannon-Weaver diversity index,Simpson dominance index,Chao and ACE richness estimation.The result showed that the bacteria of Xiaokuer Lake consists of Proteobacteria(34.5%),Bacteroidetes(27.6%),Firmicutes(24.1%),Chlorobi(3.4%),Verrucomicrobia(3.4%) and unclassified(6.9%).In the case of the bacteria of Tuzi Lake,seven major phyla were detected including Proteobacteria(51.9%),Bacteroidetes(7.4%),Firmicutes(22.2%),Acidobacteria(3.7%),Actinobacteria(3.7%),Planctomycetes(3.7%) and unclassified(7.4%).It is concluded that the bacteria of the Tuzi Lake is more diverse and rich than that of the Xiaokuer Lake by several diversity index.At last it demonstrated that lakes within Xinjiang harbor have quite diverse microorganism and are worth of further investigation.