生物
尿路上皮
分子生物学
移行细胞癌
基因产物
转染
基因
基因表达
癌症研究
污渍
细胞培养
互补DNA
北方斑点
膀胱癌
癌症
遗传学
内分泌学
泌尿系统
作者
Terence W. McGarvey,Trang B. Nguyen,John E. Tomaszewski,Frederick C. Monson,S. Bruce Malkowicz
出处
期刊:Oncogene
[Springer Nature]
日期:2001-03-01
卷期号:20 (9): 1042-1051
被引量:38
标识
DOI:10.1038/sj.onc.1204143
摘要
We have identified a novel cDNA product designated transitional epithelial response gene (TERE1), which was localized to chromosome 1p36. The TERE1 transcript (1.5 and 3.5 kb) is present in most normal human tissues including urothelium, but was reduced or absent in the majority of muscle invasive TCC tumors (22 out of 29 cases). The open reading frame encodes a protein of 338 amino acids (MW 36.8 KD). This protein is 57% homologous to a Drosophila protein called heix. We have shown by Western blotting and immuno-histochemistry with a polyclonal antibody to a specific TERE1 peptide, reduced or absent staining in muscle invasive tumors. Transfection of a sense TERE1 construct resulted in an 80–90% inhibition of cellular proliferation in two TCC cell lines and a lack of aneuploidy in the TERE1-transduced J82 cell line. These data suggest a potential role for this gene product in the progression of bladder cancer.
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