酵母
分离(微生物学)
抗体
计算生物学
钥匙(锁)
噬菌体展示
计算机科学
生物
免疫学
生物信息学
生物化学
计算机安全
作者
James A. Van Deventer,K. Dane Wittrup
出处
期刊:Methods in molecular biology
日期:2014-01-01
卷期号:: 151-181
被引量:62
标识
DOI:10.1007/978-1-62703-992-5_10
摘要
Antibodies play key roles as reagents, diagnostics, and therapeutics in numerous biological and biomedical research settings. Although many antibodies are commercially available, oftentimes, specific applications require the development of antibodies with customized properties. Yeast surface display is a robust, versatile, and quantitative method for generating these antibodies and is accessible to single-investigator laboratories. This protocol details the key aspects of yeast surface display library construction and screening.
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