Objective:To assess the relationship between peak oxygen uptake(PVO2) and ambulation distance in six-minute walk test(6MWT) among the healthy subjects.Method:Fifty one healthy subjects were recruited for.Data of pulmonary gas exchange in breathing,such as VO2,VCO2 were real-time measured with wireless remote sensing K4B2.Result:It was noticed there was a positive linear correlation between the ambulation distance and PVO2(r=0.619,P0.001= in 6MWT.The regression equation was set up(VO2/kg =0.05D-6.331,P0.001=.PVO2PVCO2,R1 were found,which suggested 6MWT was a test under anaerobic threshold.Conclusion:There was a closely positive linear correlation between the ambulation distance and PVO2 in 6MWT,which is safe,convenient and valuable for the evaluation of cardiopulmonary function.