色谱法
化学
药理学
微粒体
新陈代谢
尿
阿米替林
医学
酶
生物化学
作者
Xin Zhou,Chang Chen,Fangrong Zhang,Yang Zhang,Yu Feng,Hui Ouyang,Yong Xu,Hongliang Jiang
出处
期刊:Bioanalysis
[Newlands Press Ltd]
日期:2016-07-01
卷期号:8 (13): 1365-1381
被引量:5
标识
DOI:10.4155/bio-2016-0025
摘要
Aim: Amitriptyline is a widely used tricyclic antidepressant, but the metabolic studies were conducted almost 20 years ago using high-performance liquid chromatography coupled with ultraviolet detector or radiolabeled methods. Results: First, multiple ion monitoring (MIM)- enhanced product ion (EPI) scan was used to obtain the diagnostic ions or neutral losses in human liver microsome incubations with amitriptyline. Subsequently, predicted multiple reaction monitoring (MRM)-EPI scan was used to identify the metabolites in human urine with the diagnostic ions or neutral losses. Finally, product ion filtering and neutral loss filtering were used as the data mining tools to screen metabolites. Consequently, a total of 28 metabolites were identified in human urine after an oral administration using LC–MS/MS. Conclusion: An integrated workflow using LC–MS/MS was developed to comprehensively profile the metabolites of amitriptyline in human urine, in which five N-acetyl-l-cysteine conjugates were characterized as tentative biomarkers for idiosyncratic toxicity.
科研通智能强力驱动
Strongly Powered by AbleSci AI