Culture condition optimization for Monascus0301 producing pigment and its citrinin content measurement

Monascus pigment is a good edible crude pigment.This paper reports the experiment concerned with the prescription of cultured base and Culture Conditions optimization of Monascus 0301 producing pigment.It is concluded that the following optimum medium: 5%glucose,0.5%monosodium glutamate,0.05%ZnSO_(4)·7H_(2)O,0.1%MgSO_(4),0.5%K_(2)HPO_(4),0.5%KH_(2)PO_(4),and 0.03%MnSO_(4)·H_(2)O.The optimum culture conditions are: temperature,32℃;initial pH of culture medium,6.0,culture time,8 days.Under this condition,the monascus red pigment color value is 99.12U/mL and monascus yellow pigment color value is 97.02U/mL;elevated in 15.8% and 16.5% higher than 85.59 U/mL and 97.02U/mL of that obtained under usual conditions.Its citrinin content mensured by HPLC is less than 0.5μg/g,fulfilling the requirements.