肿瘤浸润淋巴细胞
医学
免疫疗法
肾透明细胞癌
基因敲除
PD-L1
肿瘤科
癌症研究
免疫系统
生物
肾细胞癌
免疫学
细胞培养
遗传学
作者
Chao Xu,Bolin Jia,Zhan Yang,Zhenwei Han,Zhu Wang,Wuyao Liu,Ye Cao,Yao Chen,Junfei Gu,Yong Zhang
出处
期刊:Cancers
[MDPI AG]
日期:2022-09-21
卷期号:14 (19): 4583-4583
被引量:4
标识
DOI:10.3390/cancers14194583
摘要
Background: TCIRG1, also known as V-ATPase-a3, is critical for cellular life activities through its dependent acidification. Prior to the present research, its relationship with prognostic and tumor immunity in clear cell renal cell carcinoma (ccRCC) had not yet been investigated. Methods: We assessed TCIRG1 expression in normal and tumor tissues using data from TCGA, GEO, GTEX, and IHC. We also analyzed the relationship between TCIRG1 and somatic mutations, TMB, DNA methylation, cancer stemness, and immune infiltration. We evaluated the relevance of TCIRG1 to immunotherapy and potential drugs. Finally, we explored the effect of TCIRG1 knockdown on tumor cells. Results: TCIRG1 was overexpressed in tumor tissue and predicted a significantly unfavorable clinical outcome. High TCIRG1 expression may be associated with fewer PBRM1 and more BAP1 mutations and may reduce DNA methylation, thus leading to a poor prognosis. TCIRG1 was strongly associated with CD8+ T-cell, Treg, and CD4+ T-cell infiltration. Moreover, TCIRG1 was positively correlated with TIDE scores and many drug sensitivities. Finally, experiments showed that the knockdown of TCIRG1 inhibited the migration of ccRCC cells. Conclusions: TCIRG1 may have great potential in identifying prognostic and immunomodulatory mechanisms in tumor patients and may provide a new therapeutic strategy for ccRCC.
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