Three-dimensional analysis of water dynamics in human skin by stimulated Raman scattering imaging

The stratum corneum (SC), the outermost layer of the skin, plays a vital role in providing a barrier against dry environments. In order to understand the barrier function and overall condition of the skin, it is essential to assess the ability of the SC to absorb and retain water. In this study, we used stimulated Raman scattering (SRS) imaging to investigate the threedimensional SC structure and water distribution as water was absorbed into dried SC sheets. Our findings indicate that the process of water absorption and retention is dependent on the specific sample and can be spatially heterogeneous. These results demonstrate the potential of SRS imaging in diagnosing skin conditions.