硝化作用
反硝化
异养
生物量(生态学)
环境化学
富营养化
废水
化学
氮气
环境工程
生物
环境科学
生态学
细菌
营养物
有机化学
遗传学
作者
Jinliang Chen,Liang Shen,Liang Yu,Haipeng Cao,Cuixue Chen,Guoliang Zhang,Zhi‐Kang Xu,Yinghua Lu
标识
DOI:10.1016/j.envres.2023.117595
摘要
Excessive nitrogen (N) discharged in water is a major cause of eutrophication and other severe environmental issues. Biological N removal via heterotrophic nitrification and aerobic denitrification (HN-AD) has drawn particular attention, owing to the merit of concurrent nitrification and denitrification inside one cell. However, the mechanisms underlying N transformation during HN-AD remain unclear. In the present study, the HN-AD Pseudomonas sp. Y15 was isolated to explore the N distribution and flow, based on stoichiometry and energetics. The total N removal efficiency of Y15 increased linearly with C/N ratio (in the range of 5–15) to ∼96.8%. Of this, ∼32.2% and ∼64.6% were transformed into gas-N and biomass-N, respectively. The observation of a new intracellular N metabolic bypass (NO → NO2) addressed the substantial gaseous N production during HN-AD. Regarding energetics, a large portion of the biomass-N is ascribed to the synthesis of amino acids, which consumes low energy. Finally, two novel stoichiometric equations for different N sources were proposed, to describe the overall HN-AD process. This study deepens the fundamental knowledge on HN-AD bacteria and enlightens their use in treating N-contaminated wastewater.
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