DNA
序列(生物学)
体外
计算生物学
遗传学
生物
DNA测序
计算机科学
分子生物学
作者
Jean-François Leblanc,Olivier Boulle,Émeline Roux,Jacques Nicolas,Dominique Lavenier,Yann Audic
标识
DOI:10.2144/btn-2023-0109
摘要
In the absence of a DNA template, the ab initio production of long double-stranded DNA molecules of predefined sequences is particularly challenging. The DNA synthesis step remains a bottleneck for many applications such as functional assessment of ancestral genes, analysis of alternative splicing or DNA-based data storage. In this report we propose a fully in vitro protocol to generate very long double-stranded DNA molecules starting from commercially available short DNA blocks in less than 3 days using Golden Gate assembly. This innovative application allowed us to streamline the process to produce a 24 kb-long DNA molecule storing part of the Declaration of the Rights of Man and of the Citizen of 1789 . The DNA molecule produced can be readily cloned into a suitable host/vector system for amplification and selection.
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