纳米棒
材料科学
免疫系统
抗原
体外
纳米材料
体内
佐剂
生物物理学
纳米技术
免疫学
化学
生物
生物化学
生物技术
作者
Lei Zhang,Zhihui Liang,Chen Chen,Xuecheng Yang,Duo Fu,Hang Bao,Min Li,Shuting Shi,Guang Yu,Yixuan Zhang,Caiqiao Zhang,Weiting Zhang,Changying Xue,Bingbing Sun
标识
DOI:10.1021/acsami.1c17804
摘要
Hydroxyapatite (HAP) has been formulated as adjuvants in vaccines for human use. However, the optimal properties required for HAP nanoparticles to elicit adjuvanticity and the underlying immunopotentiation mechanisms have not been fully elucidated. Herein, a library of HAP nanorods and nanospheres was synthesized to explore the effect of the particle shape and aspect ratio on the immune responses in vitro and adjuvanticity in vivo. It was demonstrated that long aspect ratio HAP nanorods induced a higher degree of cell membrane depolarization and subsequent uptake, and the internalized particles elicited cathepsin B release and mitochondrial reactive oxygen species generation, which further led to pro-inflammatory responses. Furthermore, the physicochemical property-dependent immunostimulation capacities were correlated with their humoral responses in a murine hepatitis B surface antigen immunization model, with long aspect ratio HAP nanorods inducing higher antigen-specific antibody productions. Importantly, HAP nanorods significantly up-regulated the IFN-γ secretion and CD107α expression on CD8+ T cells in immunized mice. Further mechanistic studies demonstrated that HAP nanorods with defined properties exerted immunomodulatory effects by enhanced antigen persistence and immune cell recruitments. Our study provides a rational design strategy for engineered nanomaterial-based vaccine adjuvants.
科研通智能强力驱动
Strongly Powered by AbleSci AI