拟南芥
开花位点C
生物
RNA剪接
内含子
RNA识别基序
SR蛋白
RNA结合蛋白
拼接因子
细胞生物学
核糖核酸
抑制因子
遗传学
拟南芥
选择性拼接
基因
转录因子
外显子
突变体
作者
Yu Zhang,Sheng Fan,Changmei Hua,Zhi Wei Norman Teo,Jian Xuan Kiang,Lisha Shen,Hao Yu
出处
期刊:Science Advances
[American Association for the Advancement of Science (AAAS)]
日期:2022-06-24
卷期号:8 (25)
被引量:17
标识
DOI:10.1126/sciadv.abn5488
摘要
RNA binding proteins mediate posttranscriptional RNA metabolism and play regulatory roles in many developmental processes in eukaryotes. Despite their known effects on the floral transition from vegetative to reproductive growth in plants, the underlying mechanisms remain largely obscure. Here, we show that a hitherto unknown RNA binding protein, hnRNP R-LIKE PROTEIN (HRLP), inhibits cotranscriptional splicing of a key floral repressor gene FLOWERING LOCUS C (FLC). This, in turn, facilitates R-loop formation near FLC intron I to repress its transcription, thereby promoting the floral transition in Arabidopsis thaliana. HRLP, together with the splicing factor ARGININE/SERINE-RICH 45, forms phase-separated nuclear condensates with liquid-like properties, which is essential for HRLP function in regulating FLC splicing, R-loop formation, and RNA Polymerase II recruitment. Our findings reveal that inhibition of cotranscriptional splicing of FLC by nuclear HRLP condensates constitutes the molecular basis for down-regulation of FLC transcript levels to ensure the reproductive success of Arabidopsis.
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