Optimization of chitinase production from Serratia marcescens - A classical approach

The fermentative physicochemical parameters such as agitation speed, temperature and pH were optimized by classical approach method for the production of chitinase enzyme and growth of bacterial strain of Serratia marcescens. The parameters set up for reaching maximum response for production of chitinase and cell growth were obtained at 250 rpm agitation speed, 30 oC temperature and the pH of 9. The chitinase production increased three fold using optimized culture compositions and culture conditions. The maximum production of chitinase obtained as 44.01 units/mL