Modifying the four vessel occlusion to establish ischemic brain injury coma model in rats

Objective To establish a kind of ideal ischemic brain injury coma model in rats.Methods by using four vessel occlusion and modifying four vessel occlusion add diameter of 0.45mm、0.60mm、0.70mm syringe needle to make the internal carotid artery stenosis(established by zhen hua zhou of our department)and compare the time of coma and mortality among all of the groups,to find an ideal ischemic brain injury coma model in rats.Results Modifying the four vessel occlusion add diameter 0.60mm syringe needle to make the internal carotid artery stenosis can obviously prolong the time of coma and the rat can survive,the time of coma can achieve 6～8 hours.compared with others groups are significant deviation(P0.01).Conclusion Modifying the four vessel occlusion add diameter 0.60mm syringe needle to make the internal carotid artery stenosis is suitable to establish ishemic brain injury coma model in rat.