Chemical constituents of Hypocreales sp.,an endophytic fungus derived from the red alga Symphyocladia latiuscula

From the fermentation broth of Hypocreales sp.,an endophytic fungus derived from the red alga Symphyocladia latiuscula,ten compounds were isolated by the normal phase silica gel,Sephadex LH-20 column chromatography separation,and by the preparative thin layer chromatography(PTLC) as well as by re-crystallization.Structures of purified compounds were elucidated by analysis of spectroscopic data including MS,1D and 2D NMR as bisphenol A(1),salicylic acid(2),3-indoleformic acid(3),3-indoleacetic acid(4),N-acetyltryptamine(5),22E,24R-ergosta-7,9,22-trien-3β-ol(6),ergosterol peroxide(7),and 22E,24R-5α,6α-epoxyergosta-8,22-dien-3β,7α-diol(8),ergosta-7,22-dien-6β-methoxy-3β,5α-diol(9),cerevisterol(10).All the compounds were obtained from this fungus for the first time and compound(1) was reported as a new natural product.